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Registro Completo |
Biblioteca(s): |
Embrapa Agricultura Digital; Embrapa Hortaliças. |
Data corrente: |
09/08/2022 |
Data da última atualização: |
11/08/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
MARTINS, T. P.; REGO, C. M.; NAKASU, E. Y. T.; FERNANDES, F. R.; INOUE-NAGATA, A. K. |
Afiliação: |
T. P. MARTINS, UNIVERSIDADE DE BRASÍLIA; C. M. REGO, UNIVERSIDADE DE BRASÍLIA; ERICH YUKIO TEMPEL NAKASU, CNPH; FERNANDA RAUSCH FERNANDES, CNPTIA; ALICE KAZUKO INOUE NAGATA, CNPH. |
Título: |
A high viral diversity in tomato crops in Brazil is revealed by next generation sequencing analyses. |
Ano de publicação: |
2021 |
Fonte/Imprenta: |
Acta Horticulturae, v. 1316, p. 99-105, 2021. |
ISSN: |
2406-6168 |
DOI: |
10.17660/ActaHortic.2021.1316.14 |
Idioma: |
Inglês |
Notas: |
Edition of Proceedings of the VI International Symposium on Tomato Diseases: Managing Tomato Diseases in the Face of Globalization and Climate Change. |
Conteúdo: |
Tomato is planted in Brazil mainly for fresh consumption and tomato paste production. Among the various pathogens that infect tomato plants in Brazil, viruses are particularly important due to their high incidence and the resulting losses caused. Diagnosis of viral diseases usually relies on detection methods directed to known viruses and close variants, either by serology or nucleic acid hybridization/ amplification. However, the advent of next generation sequencing (NGS) facilitated a deep analysis of viral populations, which can be used for identification, assembly and discovery of new viruses. Aiming to estimate the viral diversity present in tomato crops from three states of Brazil, five composite leaf samples were analyzed using NGS. The samples referred as Braz (collected in the Federal District, 2015); Ahol, Toca1, and Toca2 (São Paulo State, 2014), and RNY2 (Minas Gerais State, 2013) were submitted to semi-purification of viral particles and RNA extraction before RNA-seq (Illumina). The reads were filtered for quality, the contigs assembled (Velvet algorithm), and submitted to MegaBLAST analysis against a virus reference sequences database. These samples were collected from plants showing symptoms such as mosaic, chlorosis, leaf curling, chlorotic spots, necrosis and stunting. Known viruses belonging to nine genera, Crinivirus, Begomovirus, Tospovirus, Tobravirus, Potyvirus, Tobamovirus, Tymovirus, Potexvirus and Cucumovirus, were detected. Potentially undescribed and unreported viruses in tomatoes, such as an amalgavirus and an ilarvirus, were also detected and are under confirmation. The conclusion was that there is a high virus diversity present in tomato plants in Brazil, making tomato production a challenge to the growers. The crinivirus, Tomato chlorosis virus, was the most frequently found within the samplings, suggesting that it is widespread in the major tomato production areas. Two begomoviruses were detected, implying that this strategy is also useful to detect viruses with a DNA genome. Finally, this technique was particularly convenient to identify the viruses coexisting in tomatoes and to find unknown viruses that may threaten the tomato production in the country. MenosTomato is planted in Brazil mainly for fresh consumption and tomato paste production. Among the various pathogens that infect tomato plants in Brazil, viruses are particularly important due to their high incidence and the resulting losses caused. Diagnosis of viral diseases usually relies on detection methods directed to known viruses and close variants, either by serology or nucleic acid hybridization/ amplification. However, the advent of next generation sequencing (NGS) facilitated a deep analysis of viral populations, which can be used for identification, assembly and discovery of new viruses. Aiming to estimate the viral diversity present in tomato crops from three states of Brazil, five composite leaf samples were analyzed using NGS. The samples referred as Braz (collected in the Federal District, 2015); Ahol, Toca1, and Toca2 (São Paulo State, 2014), and RNY2 (Minas Gerais State, 2013) were submitted to semi-purification of viral particles and RNA extraction before RNA-seq (Illumina). The reads were filtered for quality, the contigs assembled (Velvet algorithm), and submitted to MegaBLAST analysis against a virus reference sequences database. These samples were collected from plants showing symptoms such as mosaic, chlorosis, leaf curling, chlorotic spots, necrosis and stunting. Known viruses belonging to nine genera, Crinivirus, Begomovirus, Tospovirus, Tobravirus, Potyvirus, Tobamovirus, Tymovirus, Potexvirus and Cucumovirus, were detected. Potentially undescribed a... Mostrar Tudo |
Palavras-Chave: |
HTS; Next Generation Sequencing; Sequenciamento de nova geração; Virome. |
Thesagro: |
Tomate; Vírus. |
Thesaurus Nal: |
Solanum lycopersicum. |
Categoria do assunto: |
-- |
Marc: |
LEADER 03214naa a2200289 a 4500 001 2145363 005 2022-08-11 008 2021 bl uuuu u00u1 u #d 022 $a2406-6168 024 7 $a10.17660/ActaHortic.2021.1316.14$2DOI 100 1 $aMARTINS, T. P. 245 $aA high viral diversity in tomato crops in Brazil is revealed by next generation sequencing analyses.$h[electronic resource] 260 $c2021 500 $aEdition of Proceedings of the VI International Symposium on Tomato Diseases: Managing Tomato Diseases in the Face of Globalization and Climate Change. 520 $aTomato is planted in Brazil mainly for fresh consumption and tomato paste production. Among the various pathogens that infect tomato plants in Brazil, viruses are particularly important due to their high incidence and the resulting losses caused. Diagnosis of viral diseases usually relies on detection methods directed to known viruses and close variants, either by serology or nucleic acid hybridization/ amplification. However, the advent of next generation sequencing (NGS) facilitated a deep analysis of viral populations, which can be used for identification, assembly and discovery of new viruses. Aiming to estimate the viral diversity present in tomato crops from three states of Brazil, five composite leaf samples were analyzed using NGS. The samples referred as Braz (collected in the Federal District, 2015); Ahol, Toca1, and Toca2 (São Paulo State, 2014), and RNY2 (Minas Gerais State, 2013) were submitted to semi-purification of viral particles and RNA extraction before RNA-seq (Illumina). The reads were filtered for quality, the contigs assembled (Velvet algorithm), and submitted to MegaBLAST analysis against a virus reference sequences database. These samples were collected from plants showing symptoms such as mosaic, chlorosis, leaf curling, chlorotic spots, necrosis and stunting. Known viruses belonging to nine genera, Crinivirus, Begomovirus, Tospovirus, Tobravirus, Potyvirus, Tobamovirus, Tymovirus, Potexvirus and Cucumovirus, were detected. Potentially undescribed and unreported viruses in tomatoes, such as an amalgavirus and an ilarvirus, were also detected and are under confirmation. The conclusion was that there is a high virus diversity present in tomato plants in Brazil, making tomato production a challenge to the growers. The crinivirus, Tomato chlorosis virus, was the most frequently found within the samplings, suggesting that it is widespread in the major tomato production areas. Two begomoviruses were detected, implying that this strategy is also useful to detect viruses with a DNA genome. Finally, this technique was particularly convenient to identify the viruses coexisting in tomatoes and to find unknown viruses that may threaten the tomato production in the country. 650 $aSolanum lycopersicum 650 $aTomate 650 $aVírus 653 $aHTS 653 $aNext Generation Sequencing 653 $aSequenciamento de nova geração 653 $aVirome 700 1 $aREGO, C. M. 700 1 $aNAKASU, E. Y. T. 700 1 $aFERNANDES, F. R. 700 1 $aINOUE-NAGATA, A. K. 773 $tActa Horticulturae$gv. 1316, p. 99-105, 2021.
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Embrapa Hortaliças (CNPH) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Agrobiologia. Para informações adicionais entre em contato com cnpab.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Agrobiologia. |
Data corrente: |
19/07/2019 |
Data da última atualização: |
11/09/2020 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
SANTOS, S. G. dos; RIBEIRO, F. da S.; ALVES, G. C.; SANTOS, L. A.; REIS, V. M. |
Afiliação: |
Silvana Gomes dos Santos, Universidade Federal Rural do Rio de Janeiro; Flaviane da Silva Ribeiro, Instituto Federal do Pará, Campus Bragança; Gabriela Cavalcanti Alves, UFRRJ; Leandro Azevedo Santos, UFRRJ; VERONICA MASSENA REIS, CNPAB. |
Título: |
Inoculation with five diazotrophs alters nitrogen metabolism during the initial growth of sugarcane varieties with contrasting responses to added nitrogen |
Ano de publicação: |
2019 |
Fonte/Imprenta: |
Plant and Soil, First Online, 08 May, 2019 |
Idioma: |
Inglês |
Notas: |
Publicado na Plant and Soil, v. 451, 25-44, 2020. |
Conteúdo: |
We examined the influence of inoculation with five species/strains of diazotrophic bacteria on the modulation of two enzymes involved in the assimilation of N and on the soluble N fractions in the sugarcane varieties RB867515 (adapted for low fertility soils) and IACSP95-5000 (adapted for medium to high fertility soils) under high- (3 mM) and low (0.3 mM)-N conditions in hydroponic cultivation for 59 days.The sugarcane plants were produced in three steps to obtain the hydroponic cultivation: the supply of 3 mM N for 30 days (first harvest), N depletion for 72 h (second harvest), and cultivation in high- and low-N conditions over 26 days (final harvest). Inoculation was performed by immersion of the minisetts in a diluted solution of five diazotrophic bacteria. After the final harvest, plants were divided into roots and shoots to assess their dry weight and N, P, and K accumulation.The variety played an important role in the interaction with diazotrophs, each showing distinct behavior in the activity of their N-assimilation enzymes. The nitrate reductase activity (NRa?EC 1.7.1.1) was increased in var. RB867515 by 26% in the shoots and by 48% in the roots after 72 h under N depletion, while var. IACSP95-5000 showed a reduced enzymatic activity in the roots (by 62%) but not in the shoots. Under high-N conditions, the inoculated IACSP95-5000 plants showed 31% higher glutamine synthetase activity (GSa?EC 6.31.2) compared with 19% in RB867515. Under low-N conditions, the GSas were 21% and 16% higher in the inoculated RB867515 and IACSP95-5000 plants, respectively, compared with that of the control. The content of nitrogen in the form of nitrate (N-nitrate) confirmed these varietal differences, but the soluble sugar content did not. MenosWe examined the influence of inoculation with five species/strains of diazotrophic bacteria on the modulation of two enzymes involved in the assimilation of N and on the soluble N fractions in the sugarcane varieties RB867515 (adapted for low fertility soils) and IACSP95-5000 (adapted for medium to high fertility soils) under high- (3 mM) and low (0.3 mM)-N conditions in hydroponic cultivation for 59 days.The sugarcane plants were produced in three steps to obtain the hydroponic cultivation: the supply of 3 mM N for 30 days (first harvest), N depletion for 72 h (second harvest), and cultivation in high- and low-N conditions over 26 days (final harvest). Inoculation was performed by immersion of the minisetts in a diluted solution of five diazotrophic bacteria. After the final harvest, plants were divided into roots and shoots to assess their dry weight and N, P, and K accumulation.The variety played an important role in the interaction with diazotrophs, each showing distinct behavior in the activity of their N-assimilation enzymes. The nitrate reductase activity (NRa?EC 1.7.1.1) was increased in var. RB867515 by 26% in the shoots and by 48% in the roots after 72 h under N depletion, while var. IACSP95-5000 showed a reduced enzymatic activity in the roots (by 62%) but not in the shoots. Under high-N conditions, the inoculated IACSP95-5000 plants showed 31% higher glutamine synthetase activity (GSa?EC 6.31.2) compared with 19% in RB867515. Under low-N conditions, the GSas were... Mostrar Tudo |
Palavras-Chave: |
Diazotrophic bacteria; Enzymatic activity; Saccharum sp. |
Thesaurus NAL: |
Nitrogen. |
Categoria do assunto: |
S Ciências Biológicas |
Marc: |
LEADER 02516naa a2200229 a 4500 001 2110764 005 2020-09-11 008 2019 bl uuuu u00u1 u #d 100 1 $aSANTOS, S. G. dos 245 $aInoculation with five diazotrophs alters nitrogen metabolism during the initial growth of sugarcane varieties with contrasting responses to added nitrogen$h[electronic resource] 260 $c2019 500 $aPublicado na Plant and Soil, v. 451, 25-44, 2020. 520 $aWe examined the influence of inoculation with five species/strains of diazotrophic bacteria on the modulation of two enzymes involved in the assimilation of N and on the soluble N fractions in the sugarcane varieties RB867515 (adapted for low fertility soils) and IACSP95-5000 (adapted for medium to high fertility soils) under high- (3 mM) and low (0.3 mM)-N conditions in hydroponic cultivation for 59 days.The sugarcane plants were produced in three steps to obtain the hydroponic cultivation: the supply of 3 mM N for 30 days (first harvest), N depletion for 72 h (second harvest), and cultivation in high- and low-N conditions over 26 days (final harvest). Inoculation was performed by immersion of the minisetts in a diluted solution of five diazotrophic bacteria. After the final harvest, plants were divided into roots and shoots to assess their dry weight and N, P, and K accumulation.The variety played an important role in the interaction with diazotrophs, each showing distinct behavior in the activity of their N-assimilation enzymes. The nitrate reductase activity (NRa?EC 1.7.1.1) was increased in var. RB867515 by 26% in the shoots and by 48% in the roots after 72 h under N depletion, while var. IACSP95-5000 showed a reduced enzymatic activity in the roots (by 62%) but not in the shoots. Under high-N conditions, the inoculated IACSP95-5000 plants showed 31% higher glutamine synthetase activity (GSa?EC 6.31.2) compared with 19% in RB867515. Under low-N conditions, the GSas were 21% and 16% higher in the inoculated RB867515 and IACSP95-5000 plants, respectively, compared with that of the control. The content of nitrogen in the form of nitrate (N-nitrate) confirmed these varietal differences, but the soluble sugar content did not. 650 $aNitrogen 653 $aDiazotrophic bacteria 653 $aEnzymatic activity 653 $aSaccharum sp 700 1 $aRIBEIRO, F. da S. 700 1 $aALVES, G. C. 700 1 $aSANTOS, L. A. 700 1 $aREIS, V. M. 773 $tPlant and Soil, First Online, 08 May, 2019
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